Long read sequencing has been needed for microbiome and metagenome sequencing .. in the past low quality of reads and high price has hindered acceptance of the available long read sequencing. MR DNA http://www.mrdnalab.com has pushed back these restrictions and now offer superior quality sequencing with low error rates .. MR DNA has a wide variety of primers available for amplicon sequencing .. a range of possible 16s, ITS1-4, 18s, functional genes and the routine ability to use any custom primer > 500bp that the customer chooses..
16s long read 27F-1492R sequencing captures all 9 variable regions of the 16s gene.. this is performed using the bTEFAP(R) service model on Pac Bio Sequel II. lower cost and higher quality of the data ensure that microbiome studies can capture bacteria and improvements in these long reads allows species level classifications with confidence in ability to publish microbiome data comparisons that are no longer tentative as it has been with other powerful technologies such as illumina. Illumina still has lower cost and ability to classify the microbiome taxonomically but species level classifications are tentative.. the MR DNA service with long read sequencing of the 16s gene for bacteria and now new selective V1-V9 assays for archaea have revolutionized the microbiome science allowing species level calls.
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the ITS1-4 assay for fungal classification is an assay that can evaluate the species of fungi .. this gene region because it has an average size range of roughly 500bp up to 1600bp has been with short read sequencing replaced by the very short 280bp ITS1-2 region.. this is no longer a limitation with the long read sequencing as it can easily cover the entire range of this ITS1-4 amplicon. This ability to evaluate the fungal mycobiome on pac bio sequel was brought out by MR DNA in the early days of the pac bio sequel and now with improvements in this technology the lower price and higher volumes of data MR DNA can produce make this the best choice for evaluating the mycobiome in any sample type.
the 18s gene is a great university marker for evaluating the eukaryote content of any sample.. with the short read technologies we are limited to fragments of up to 500bp typically.. especially with the 18s gene this small fragment has very limited ability to provide good taxonomic classifications because of the low rate of variation in the 18s gene in such small sequences. Now we have the ability to sequence 1000 up to 2200bp of the 18s gene .. even covering the 18s gene the ITS gene and into the large subunit 23S .. such long reads improve the resolution of the taxonomic classification for eukaryotes .. MR DNA has tested a wide range of 18s genes and their service for 18s eukaryote biome sequencing can dramatically improve your research capabilities.
contact MR DNA through their website at http://www.mrdnalab.com